I get especially chapped out by Followers - or those whom I'm Following - who won't interact with you.  There's little point in Tweeting your heart out if you never get a shred of validation from anyone else that your words were read, much less meant something.  Where's the conversing?

Be supportive, folks!  Doesn't take much to give other Tweeps a little feedback!  Let 'em know that their shouts aren't just falling on deaf ears.